TABLE 1.
Comparison of EIAs for the detection of WNV IgM antibodies in the serum of experimentally infected monkeysa
| Monkey | Day monkey was identified as WNV IgM positive by:
|
|||
|---|---|---|---|---|
| COS-1 cell-based assay
|
S2 cell-based assay
|
|||
| First | Last | First | Last | |
| J840 | 12 | 28 | 13b | 21 |
| R470 | 11 | 45 | 11 | 45 |
| T810 | 10c | 63 | 10 | 63 |
| P469 | 9 | 63 | 9 | 63 |
| P659 | 9 | 45 | 10 | 28 |
Serum samples were collected on days 0, 1 to 14, 21, 28, 45, and 63 postinfection. All animals were euthanized on day 63 postinfection except for monkey R470, which was euthanized on day 45.
For monkey J840, the S/N ratio as determined by the S2 assay for the sample obtained on day 12 was 4.10, and the cutoff S/N ratio was 4.20.
For monkey T810, the sample obtained on day 12 was WNV RNA positive as determined by reverse transcription-PCR (15).