TABLE 3.
IgM reactivity (expressed as S/N ratios) to COS-1 and S2 cell-expressed WNV antigens in a panel of 27 human CSF specimensa
| Specimen | S/N ratio obtained by indicated assay:
|
Diagnosisd | ||
|---|---|---|---|---|
| COS-1b | S2b | MDCHc | ||
| 10 | 74.1 | 74.1 | 7.3 | E |
| 14 | 74.1 | 74.1 | 6.4 | M/E |
| 15 | 74.1 | 68.2 | 5.3 | |
| 17 | 74.1 | 74.1 | 5.7 | HR |
| 18 | 74.1 | 68.9 | 4.8 | M/E |
| 20 | 74.1 | 32.2 | 3.6 | E |
| 23 | 74.1 | 74.1 | 5.8 | |
| 24 | 74.1 | 57.9 | 3.5 | E |
| 25 | 74.1 | 65.9 | 6.4 | M |
| 26 | 74.1 | 74.1 | 5.8 | E |
| 28 | 74.1 | 74.1 | 5.9 | E |
| 30 | 74.1 | 74.1 | 6.4 | M/E |
| 31 | 74.1 | 74.1 | 5.3 | |
| 33 | 74.1 | 74.1 | 4.3 | E |
| 8 | 74.1 | 74.1 | 7.0 | |
| 9 | 74.1 | 74.1 | 5.6 | |
| 32 | 71.9 | 32.8 | 5.0 | H |
| 22 | 71.4 | 49.6 | 6.4 | E |
| 19 | 58.4 | 41.1 | 5.5 | E |
| 27 | 36.8 | 28.7 | 3.3 | M |
| 12 | 29.7 | 15.1 | 4.7 | E |
| 21 | 13.8 | 8.6 | 1.9 | M |
| 34 | 8.1 | 4.8 | 1.0 | |
| 16 | 3.5 | 2.5 | 1.4 | M |
| 11 | 1.7 | 2.4 | 1.7 | |
| 35 | 1.4 | 1.1 | 1.0 | E |
| 13 | 1.3 | 1.7 | 1.3 | E |
a
Reactivity for all three assays is reported as S/N ratios. Tabular data are arranged according to S/N ratios from the COS-1 assay, listed from highest to lowest. Samples were diluted 1:5 in assay buffer prior to testing.
b
COS-1 and S2 S/N ratios represent the means of duplicate determinations.
c
For results obtained at the MDCH, samples with S/N ratios of <2.0 are considered negative, those with S/N ratios from 2.0 to 10.0 are equivocal, and those with S/N ratios of >10.0 are positive for WNV IgM.
d
Clinical diagnosis abbreviations: see footnote d of Table 2.