Table 1.
Strains and plasmids used in this study.
| Strain | Relevant features or genotype | Construction, source or reference |
|---|---|---|
| B. subtilis | ||
| 168 | trpC2 | Laboratory stock |
| BSB1 | trp+ | Nicolas et al., 2012 |
| 1356 | zapA-yshB::tet | Feucht and Errington, 2005 |
| 2020 | amyE::(Pxyl-gfpmut1-ftsZ, spc) | J. Sievers (unpublished) |
| 3362 | ezrA::tet | Hamoen et al., 2006 |
| 3828 | ftsL::pSG441 aphA-3 Pspac-pbpB, amyE::cat Pxyl-Δ30-ftsL | Bramkamp et al., 2006 |
| 4077 | ylmBC::(erm Pspac-ylmD), ezrA::tet | Hamoen et al., 2006 |
| 814 | ΔftsL-Pspac-pbpB kan, amyE::Pxyl-HA-ftsL cat | Daniel and Errington, 2000 |
| BG239 | thr-5, tet-4 | Wei and Bechhofer, 2002 |
| KS273 | aprE::Pspac-zapA spc | Surdova et al., 2013 |
| LH28 | ezrA::cat | L. Hamoen (unpublished) |
| SG82 | lacA::tet | Laboratory stock |
| YK012 | CRK6000 ezrA::spc | Kawai and Ogasawara, 2006 |
| YK204 | CRK6000 sepF::spc | Ishikawa et al., 2006 |
| PG49 | ezrA::spc | YK012 DNA → 168 |
| PG100 | lacA::tet, ezra::cat | LH28 DNA → SG82 |
| PG112 | tet-4 | 5kb rpsJ region from BG239 → 168 |
| PG113 | tet-4, ezrA::spc | 5kb rpsJ region from BG239 → PG49 |
| PG116 | tet-4, ezrA::cat | 5kb rpsJ region from BG239 → LH28 |
| PG121 | ezrA::tet, tseB:TnYLB-1 kan | pMarB integration into 3362 |
| PG126 | ezrA::tet, zapA-TnYLB-1- yshB kan | pMarB integration into 3362 |
| PG129 | ezrA::tet, galE:TnYLB-1 kan | pMarB integration into 3362 |
| PG135 | tseB:TnYLB-1 kan | PG121 DNA → 168 |
| PG140 | zapA-TnYLB-1- yshB kan | PG126 DNA → 168 |
| PG143 | galE:TnYLB-1 kan | PG129 DNA → 168 |
| PG149 | aprE::Pspac-zapA, spc | KS273 DNA → 168 |
| PG158 | sepF::spc | YK204 DNA → 168 |
| PG160 | ezrA::tet, amyE::Pxyl-Δ30ftsL-cat | 3828 DNA → 3362 |
| PG162 | ezrA::cat, aprE::Pspac-zapA spc | PG149 DNA → LH28 |
| PG164 | ezrA::cat, zapA-yshB::tet, Pspac-zapA | 1356 DNA → PG162 |
| PG209 | ezrA::tet, amyE::Pxyl-gfp-ftsZ spc | 2020 DNA → 3362 |
| PG234 | galE::kan | This work |
| PG235 | tseB::kan | This work |
| PG238 | ezrA::tet, galE::kan | PG234 DNA → 3362 |
| PG239 | ezrA::tet, tseB::kan | PG235 DNA → 3362 |
| PG251 | galE::spc | This work |
| PG252 | tseB::spc | This work |
| PG294 | ezrA::tet, galE::kan, sepF::spc | YK204 DNA → PG238 |
| PG296 | galE::kan, ylmBC::(erm Pspac-ylmD), ezrA::tet | 4077 DNA → PG238 |
| PG305 | ΔftsL-Pspac-pbpB kan, amyE::Pxyl-HA-ftsL cat, ezrA::tet | 3362 DNA → 814 |
| PG307 | ezrA::cat, zapA-yshB::tet, aprE::Pspac-zapA spc, galE::kan | PG234 DNA → PG164 |
| PG325 | aprE::Pspac-tseB spc | pPG16 → 168 |
| PG327 | aprE::Pspac-galE spc | pPG18 → 168 |
| PG330 | tseB::kan, aprE::Pspac-tseB spc | PG325 DNA → PG235 |
| PG332 | ezrA::tet, galE::kan, aprE::Pspac-galE spc | PG327 DNA → PG239 |
| PG333 | ezrA::tet, tseB::kan, aprE::Pspac-tseB spc | PG325 DNA → PG239 |
| PG718 | trp+, amyE::Pxyl-gfp-tseB spc | pPG6(mGFP) integration into BSB1 |
| PG742 | ΔftsL-Pspac-pbpB kan, amyE::Pxyl-HA-ftsL cat, lacA::tet | SG82 DNA → 814 |
| E. coli | ||
| DH5α | F−, φ80lacZΔM15, Δ(lacZYAargF)U196, recA1, endA1, hsdR17, (rK−, mK+), phoA, supE44, λ−, thi−1, gyrA96, relA1 | Invitrogen |
| Plasmid | Relevant features or genotype | Construction, source or references |
| pAPNC213 | bla aprE' spc lacI Pspac'aprE | Morimoto et al., 2002 |
| pMarB | bla erm Pctc Himar1 kan (TnYLB-1) | Le Breton et al., 2006 |
| pSG1729 | bla amyE3' spc Pxyl-gfpmut1' amyE5' | Lewis and Marston, 1999 |
| pHT21 | kan | Trieu-Cuot and Courvalin, 1983 |
| pLOSS* | spc | Claessen et al., 2008 |
| pPG6(mGFP) | bla amyE3' spc PxyltseB-mgfpm1' amyE5' | This work |
| pPG16 | bla aprE' spc lacI Pspac tseB'aprE | This work |
| pPG18 | bla aprE' spc lacI Pspac galE'aprE | This work |
Unless stated otherwise, all strains were made in the 168 wild type background. Genes responsible for resistance to antibiotics are abbreviated as follows: bla, ampicillin; cat, chloramphenicol; erm, erythromycin; kan, kanamycin; spc, spectinomycin; tet, tetracycline.