FIG. 3.

Lymphocytes produce IFN-γ in response to PAF. Nonadherent PBL (primarily lymphocytes) were cultured overnight with various doses of PAF, and cell-free supernatants were harvested and screened for IFN-γ. Cytokine levels were normalized for protein recovery, and the data are presented as n-fold induction of cytokine over control (cells cultured in DMSO, the vehicle). The data shown are compiled from three independent experiments with lymphocytes from different individuals. PAF induced lymphocytes to produce IFN-γ in a dose-dependent manner (P < 0.05).