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. 2015 Apr 22;3:30. doi: 10.3389/fchem.2015.00030

Figure 6.

Figure 6

FADS/LSD1 interaction as revealed by immunological techniques. In (A) the dot-blot assay is reported: briefly, purified rat liver nuclei (pNcl, 50 or 100 μg) resuspended in the nuclear buffer or 6His-hFADS2 (5 μg) were dotted onto a nitrocellulose membrane. Where indicated pNcl (50 or 100 μg), homogenate (homo, 50 or 100 μg) or the nuclear buffer (none) were added to the dotted membrane. Protein/protein interaction was revealed immunochmically as described in Materials and Methods. In (B) rat liver homogenate (homo, 25 μg), nuclei (pNcl, 25 μg) and the immunoprecipitate (IPanti−FADS) from nuclear proteins (50 μg) were analyzed by immunoblotting with anti-FADS antiserum, as described in Materials and Methods. The same PVDF membrane was analyzed with the anti-LSD1 and anti-ACT1 antibodies after stripping procedure.