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. 2015 Apr 22;6:56. doi: 10.3389/fendo.2015.00056

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Copyright © 2015 Ma, Morshed, Latif and Davies.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of PAX8, NKX2-1, and Pax8⁺NKx2-1⁺ mouse iPS cell lines. (A) RT-PCR analysis of selected iPS cell lines for thyroid transcription factors. The figure shows PAX8, NKX2-1, and FOXE1 transcription after culture in selection medium for up to 4 weeks. Data presented (mean) are from three independent experiments in which all sample sets were analyzed in triplicate. **p < 0.01 compared to untransfected iPS cells. (B–D) Immunofluorescence analysis of PAX8 and NKX2-1 in the transfected cell lines. Scale = 100 μm. (E) Retention of stemness in transfected cell lines is shown by the continued expression of Oct4, Sox2, Nanog, and Rex1 gene expression.