Figure 3.

Pathologic changes in the corneas of allogeneic HSCT recipients. (A) Photographs (H&E stained, 7-μm sections, ×20) of the central corneas of mice receiving B6 TCD-BM only or together with B6 T cells. Dense cellular infiltrates (black arrow) are observed in the eyes of animals with systemic GVHD. (B) Immunofluorescent microscopy. Photographs of 7-μm sections at ×40 stained with CD11b (macrophages), Ly6G (neutrophils), CD4, CD8 (T cells), mAbs (red), and DAPI (blue). Cellular infiltrates in animals with GVHD consist of T cells, macrophages, and neutrophils.