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. 2015 Apr 10;15:250. doi: 10.1186/s12885-015-1291-0

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© Shakibaei et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Light microscopic demonstration of HCT116 (A-D) and HCT116R (E-H) cells (1 × 10⁶/ml) grown in alginate beads culture. Day 1–3 of cultures (A;E), encapsulated cells revealed cell aggregates, the typical spherical shape of HCT116 and HCT116R (arrows). During the following 7–10 days (B;F), HCT116 and HCT116R cells formed large spheroids and were placed to the channel exterior (arrowheads) when the alginate bead surface was ruptured. With time, days 14–21 days, HCT116 (C-D) and HCT116R (G-H) cells aggregates enlarged and more and more cells migrated from the beads. x24, bar=0.2 mm in all cases.