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. 2015 Apr 10;15:250. doi: 10.1186/s12885-015-1291-0

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© Shakibaei et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The emigration behavior of HCT116 and HCT116R cells in alginate 3D culture. Toluidine blue staining (A) and quantitative evaluation of the spheroid number (B) emigrated through alginate beads during the culture period from day 1–36.