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. 2015 Apr 15;128(8):1580–1594. doi: 10.1242/jcs.166447

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — FAZ proteins are stably associated with the cytoskeleton and colocalise with FAZ markers.(A) Cytoskeletons from cells expressing FAZ–eYFP proteins. The left images show the eYFP signal and the merged images are phase overlaid with the eYFP (green) signal. Arrows indicate regions where the flagellum is detached and the eYFP signal remains with the cell body. (B,C) Cytoskeletons expressing FAZ–eYFP proteins (green) stained with FAZ1 and DOT1 antibodies (red) respectively. Scale bars: 2 µm.