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. 2015 Apr 15;128(8):1580–1594. doi: 10.1242/jcs.166447

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — New FAZ proteins are added at the proximal end of the assembling FAZ.(A) 2FAZ-cytoskeletons at 2 and 4 hours post induction with phase, the FAZ–eYFP protein signal and merged images [phase overlaid with FAZ–eYFP protein (green) and DAPI (blue)] shown. Scale bars: 2 µm. (B) Cartoons showing the different FAZ–eYFP signals observed in 2FAZ-cytoskeletons. The top line is the new FAZ and the bottom line is the old FAZ with black marking unlabelled FAZs and green marking FAZ–eYFP-labelled FAZs. (C) Graphs showing the number of each cytoskeleton category observed for 2FAZ-cytoskeletons at 0, 2, 4 and 8 hours after induction (n = 250 from three independent experiments).