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. 2015 Apr 15;128(8):1580–1594. doi: 10.1242/jcs.166447

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Newly synthesised FLA1BP protein is predominantly added at the proximal end of the FAZ and the new PFR2 protein is added at the distal end of the flagellum.(A) 2FAZ-cytoskeleton after 4 hours of doxycycline induction with phase, FLA1BP–eYFP (FLA1BP::eYFP) signal and merged images [phase overlaid with FLA1BP–eYFP (green) and DAPI (blue)] shown. The old FAZ has a proximal signal only (white asterisk). (B) 2FAZ-cytoskeleton after 8 hours of doxycycline induction. The old FAZ has a proximal (white asterisk) and distal signal (white arrow). (C) 1FAZ-cytoskeleton after 8 hours of doxycycline induction. The FAZ has a proximal (white asterisk) and distal signal (white arrow). Scale bars: 2 µm. (D) Cartoons showing the different FLA1BP–eYFP signals observed in 2FAZ-cytoskeletons. The top line is the new FAZ and the bottom line is the old FAZ with black marking unlabelled FAZs and green marking FLA1BP–eYFP-labelled FAZs. (E) Graph showing the number of each cytoskeleton category observed for 2FAZ-cytoskeletons at 0, 2, 4 and 8 hours after induction (n = 250 from three independent experiments). (F) Cytoskeletons showing the distinct localisation of FAZ1 and PFR2 in the same cell with phase, the eYFP–FAZ1 signal, the PFR2–dTomFP (PFR2::dTomFP) signal and merged images [phase overlaid with eYFP–FAZ1 (green) and PFR2–dTomFP (red)] shown. Scale bars: 2 µm.