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. 2015 Apr 15;128(8):1607–1616. doi: 10.1242/jcs.167817

Fig. 3.

Fig. 3.

Luciferase reporter activity indicates direct binding and regulation of miRNAs.Luciferase reporter activity indicates direct binding and regulation of miRNAs. (A) Sequences of Renilla or firefly luciferase under regulation of CDKN1A or KCNK3 3′UTRs that were used for transient reporter assay experiments. Wild-type (WT) and mutant (mut) alleles for each of the three miRNA-binding sites are presented. The miRNA seed region and complimentary 3′UTR sequence are marked in bold. Mutagenized nucleotides are in red and bold. (B) Luciferase activity at 48 hours after co-transfection of different miRNA combinations with Renilla and firefly luciferase constructs under regulation of the CDKN1A or TASK1 3′UTR. The data presented are the relative levels of Renilla luciferase expression standardized to those of firefly luciferase. Data show the mean±s.e.m. (n = 3); *P<0.05 (paired Student's t-test).