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. 2015 Apr 15;128(8):1607–1616. doi: 10.1242/jcs.167817

Fig. 4.

Fig. 4.

The p21 protein is regulated by let-7.The p21 protein is regulated by let-7. Flow cytometric analysis of p21 expression following 72 hours of let-7c overexpression (OE) in the Jurkat cell line, (A) sample result and (B) quantification of three experiments. (C) Cell cycle is affected by let-7 and p21 levels. At 4 days following let-7c overexpression in the Jurkat cell line, anti FITC-BrdU and 7-AAD were used in order to determine the proportion of cells in each cell cycle phase. Analysis was performed using Flowing Software 2 (Cell Imaging Core, Turku Centre for Biotechnology, Finland). The S:G1 and G2:G1 ratios in cells treated with let-7c or control are presented. Data in B,C show the mean±s.e.m. (n = 3); *P<0.05 (paired Student's t-test).