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. 2014 Dec 15;206(3):965–971. doi: 10.1111/nph.13207

Figure 2.

Figure 2

The phosphorylation state of Arabidopsis thaliana phyB S86 modifies nuclear localization and signaling when low Pfr : Ptot ratio is available. (a) Cotyledon area of wild-type Columbia (Col), phyB-9 mutant (phyB-9) and transgenic seedlings expressing either 35S:PHYB-YFP (phyB-YFP) or 35S:PHYBS86A-YFP (phyBS86A-YFP) or 35S:PHYBS86D-YFP (phyBS86D-YFP) transgenes in phyB-9 background is shown. The seedlings were grown for 4 d under 10 μmol m2 s−1 FR light. n > 30; error bars, ± SE; ***, P < 0.001, compared with phyB-9 mutant. (b) Intracellular localization of wild-type or mutant phyB-YFP chimera proteins. Confocal laser scanning microscopy images of hypocotyl cells were taken after 4 d of growth in darkness (D) or under 50 μmol m2 s−1 red light (R) or 10 μmol m2 s−1 far-red light (FR). Bar, 75 μm.