Figure 5.

Molecular analysis of T₀ transgenic plants. (A) PCR confirmation of TCON transgenic pepper plants using BamHI coxIVF and SacI orf507R primers that would amplify 693 bp fragment of the cassette in DNA level.(B) RT-PCR analysis of buds from TCON transgenic plants with Kpn I orf507F and SacI orf507R primers that would amplify 507 bp fragment with caubi gene as reference gene. (C) qRT-PCR analysis of buds from TCON transgenic plants. TCON1~8, transgenic TCON plants; WT, transformed control plant; CK, positive control (amplified from plasmid DNA); Leaf, leaves collected from the three transformants; M, Trans2K plus marker. Statistically significant differences between the means were determined using Fisher's LSD test (p < 0.05).