Figure 3.
Impact of AcrAB production on growth in the presence of styrene, 1-hexene, 1-octene, and 1-nonene. A: Different concentrations of styrene were added to an OD600 = 0.2 culture of E. coli K12 ΔacrAB containing the pABc (white) or the control plasmid p0c (black). OD was monitored after 2 h of growth at 37 °C. The bars represent standard deviation between duplicates. B: Survival tests in presence of 1-hexene, 1-octene, or 1-nonene saturated atmosphere were performed. Six different dilutions of cultures (10 cells to 107 cells, left to right) were spotted in triplicate on an agar plate before exposure to chemicals.