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Impact of AcrA, AcrB, and TolC over-production on 1-hexene tolerance. A survival test of E. coli K12 wild type containing pABc or p0c and either pTolCa or p0a, in an 1-hexene saturated atmosphere was performed. Six different dilutions of cultures (10 cells to 107 cells, top to bottom in each of the four panels) were spotted in triplicate on an agar plate. Plates were then exposed to 1-hexene (Fig. 1B).
