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Impact of AcrB mutations on 1-hexene tolerance. A: Survival test of E. coli strain producing either the AcrB wild type or variants in a 1-hexene saturated atmosphere was performed. Two dilutions of cultures (Dilution 1: 105 cells and Dilution 2: 104 cells) were spotted in triplicate on an agar plate. B: Localization of the six beneficial mutations represented in red on the structure of the AcrB trimer or C: on the schematic representation of the AcrB trimer.
