Figure 7.

Impact of AcrB mutations on growth in the presence of 1-hexene, styrene, and bile salts. Different concentrations of 1-hexene (A), styrene (B), or bile salts (C) were added to an OD₆₀₀ = 0.2 culture of E. coli K12 ΔacrAB containing either the AcrB wild type (pABc) or select AcrB variants. OD was monitored after 2.5 h, 2 h, and 1.5 h of culture at 37 °C for 1-hexene, styrene, and bile salts, respectively. Data show an average of biological replicates; triplicates for 1-hexene and bile salts, and duplicates for styrene.