FIG. 4.

Detection of B. pertussis by the microplate molecular velcro assay with the homosandwich ELISA format. The plates were coated with 1H2P4 and detected with HRPO bsMAb P123-2. The points are the means of pentuplicate measurements of each point and SDs. (Inset) Detection of B. pertussis in the low range. These are essentially the same data shown outside the inset but more clearly show the counts from 0 to 20 CFU. All points for the bacteria are significantly different from the control values by Student's t test (P < 0.001).