FIG. 5.

Direct detection of B. pertussis on nasopharyngeal swabs spiked with B. pertussis. Calgiswabs were allowed to adsorb 20 μl of sample containing various amounts of B. pertussis in an Eppendorf tube at room temperature for 5 min and dried. The swabs were fixed with 20 μl of 95% ethanol at room temperature for 5 min and blocked with 5% BSA in PBS at room temperature for 5 min. The swabs were incubated with 50 μl of bsMAb-HRPO complex at 3 μg/ml for 15 min, washed eight times with 0.05% BSA in PBS, and centrifuged at 14,000 rpm (MSE Mistral 2000) for 20 s. TMB substrate (100 μl) was added to observe color development.