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. 2015 Apr 18;8:235. doi: 10.1186/s13071-015-0844-z

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© Yang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Validation of gene expression by quantitative RT-PCR. Five immune-relevant genes were randomly selected for qPCR validation. The values are the mean of gene expression levels performed three times, as calculated by the △Ct method, normalized to beta-actin expression, and comparing the 3d, 30d, 60d versus 0d of each sheep respectively. A similar degree of variability in expression profiles between microarray and qPCR was observed, only with IGLL1 opposite in 3d vs. 0d comparison. Opposite expression between qPCR and microarray was marked by an asterisk.