Fig. 1.
Host-range restriction in Sinorhizoboim meliloti is unstable. A, Strain-host combinations evaluated in this study. Symbiotic phenotypes were scored approximately 30 days postinoculation (dpi). Fix+, effective N-fixing pairs; Fix−, abortively nodulating pairs; Fix− *, pairs exhibiting suppressible incompatibility. LU = Medicago lupulina; IT = M. italica; PR = M. praecox; A17 = M. truncatula cv. A17; and A20 = M. truncatula cv. A20. B, Representative shoot and nodule images from compatible and incompatible pairs, 30 dpi. C, Examples of gain-of-compatibility (GOC) nodules (arrows) among incompatible nodules (arrowheads) on plants originally inoculated with single strains. D, Quantification of shoot dry mass 40 dpi with strain C017 (black; n = 8), C017-GOC (gray; n = 8), or no inoculation (white; n = 16). Error bars represent standard error of the mean. E, Representative shoot and nodule images of B800 and B800-GOC inoculated onto M. truncatula cvs. A17 and A20, 30 dpi. Note that both strains are Fix+ on A17 but only B800-GOC is Fix+ on A20. F, Representative shoot and nodule images of C377 and C377-GOC inoculated onto M. truncatula cvs. A17 and A20, 30 dpi. Note that both strains are Fix− on A17 but only C377-GOC is Fix+ on A20.