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. Author manuscript; available in PMC: 2015 Apr 22.
Published in final edited form as: Mol Plant Microbe Interact. 2012 Aug;25(8):1026–1033. doi: 10.1094/MPMI-02-12-0052-R

Fig. 4.

Fig. 4

Abortive nodulation is governed by autonomously functioning accessory plasmids. A, Diagram of the process used to introduce exogenous HR plasmids (large accessory plasmids affecting symbiotic host range) into plasmid-naive strains. B, Eckhardt gels showing B464 harboring different exogenous HR plasmids. C, Representative shoot and nodule phenotypes from plants inoculated with B464 with or without pHRC017 (30 days posinoculation [dpi]). D, Symbiotic outcomes on multiple hosts after inoculation with B464 harboring different HR plasmids. E, Quantification of shoot dry mass 40 dpi with strain B464 (black; n = 8), B464 and pHRC017 (gray; n = 8), or no inoculation (white; n = 16). Error bars represent standard error of the mean. All HR plasmids referred to in B, C, D, and E are modified with the oriT/neo cassette to permit transfer and selection. All recipient strains referred to in B, C, D, and E were modified with the tet cassette to permit selection of transconjugants.