Figure 1.
Dose-dependent effect of FTC (A) and GF120918 (B) on the efflux of [3H]-MTX by MDCKII-BCRP cells. Monolayers of SV40-HCEC were incubated with [3H]-MTX (0.05 μCi/ml) under ATP-depleting conditions, and complete medium was added with and without inhibitor. The medium was analyzed after 1 hr. Each data point is representative of mean ± SD of three determinations. Statistical significance was tested by two-factor ANOVA. *p ≤ 0.05; **p ≤ 0.01.