Table 3.
Paralog and ortholog divergence in the P. vivax msp3 gene family
| Gene | n | dA Between Pv Paralogs (SE) |
dA Between Pv and Pc Orthologs (SE) Orthologs (SE) |
dA Between Pv and Pc | |||
|---|---|---|---|---|---|---|---|
| msp3D | 18 | PvD1 vs. PvD2 | 0.038 (0.003) | PvD1 vs. PcD | 0.091 (0.005) | PvD2 vs. PcD | 0.092 (0.005) |
| msp3E | 25‡ | PvE1 vs. PvE2 | 0.007 (0.001) | PvE1 vs. PcE | 0.086 (0.005) | PvE2 vs. PcE | 0.085 (0.005) |
| msp3F | 26 | PvF1 vs. PvF2 | 0.024 (0.002) | PvF1 vs. PcF | 0.089 (0.005) | PvF2 vs. PcF | 0.091 (0.005) |
Divergence between P. vivax (Pv) isolates of the three highly similar paralog pairs msp3D1-D2, msp3E1-E2, and msp3F1-F2 and each paralog’s net divergence from its respective P. cynomolgi (Pc) ortholog are shown along with standard error (SE) estimates from 1000 bootstrap replicates. Sites with alignment gaps were excluded from analysis. The number of P. vivax and P. cynomolgi sequences (n) analyzed is shown.
For msp3E, only the 16 sequences from the 8 P. vivax isolates (Salvador I, Sumatra I, Thai III, two field isolates from Thailand, and three from Venezuela) for which exactly two divergent clones of msp3E were sequenced are included with the 9 P. cymomolgi msp3E sequences. Two Thai field isolates resulted in the amplification of more than two divergent clones of msp3E; these sequences are not included as it is not clear if this was a result of mixed infection.