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. 2015 Feb 4;24(10):2966–2984. doi: 10.1093/hmg/ddv035

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© The Author 2015. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Chromatin conformation capture and reporter gene analysis of SNPs rs10816625 and rs13294895. (A) Chromatin interaction data from HindIII 3C libraries generated using MCF7 cells that indicates interactions between a fragment containing rs10816625 and rs13294895 (dashed line) and fragments surrounding KLF4. Results from three replicate libraries are plotted; each quantitative PCR reaction was performed in triplicate. Error bars represent standard mean errors. (B) Chromatin interaction data from HindIII 3C libraries generated using SUM44 cells. (C) Dual luciferase assays for reporter constructs containing the common alleles of both rs10816625 and rs13294895 (pGL4minP-AB), risk allele of rs10816625 (pGL4minP-aB), risk allele of rs13294895 (pGL4minP-Ab) and risk alleles of both SNPs (pGL4minP-ab) transiently transfected into MCF7 cells. Ratios were normalised to a minimal promoter construct (pGL4minP). Each transfection was repeated five times and constructs were generated in both forward and reverse orientations. (D) Dual luciferase assays for reporter constructs containing the common alleles of both rs10816625 and rs13294895 (pGL4minP-AB), risk allele of rs10816625 (pGL4minP-aB), risk allele of rs13294895 (pGL4minP-Ab) and risk alleles of both SNPs (pGL4minP-ab) transiently transfected into T47D cells.