Skip to main content
. Author manuscript; available in PMC: 2015 Apr 22.
Published in final edited form as: Methods Mol Biol. 2014;1155:163–172. doi: 10.1007/978-1-4939-0669-7_14

Fig. 4.

Fig. 4

Reprehensive examples of solid-phase assay. (a) Binding of PGRN to TNFR2. Various doses of PGRN or BSA, as indicated, were absorbed to an ELISA plate. After blocking biotin-labeled TNFR2 was added, and the bound protein from the liquid phase was then detected by streptavidin conjugated with horseradish peroxidase. (b) PGRN inhibition of TNFα binding to TNFR2. After blocking, plates precoated with TNFR2 were incubated with various amounts of PGRN, as indicated, and biotin-labeled TNFα, and the bound biotin-labeled TNFα was detected as described in (a)