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. 2015 Apr 22;10(4):e0123702. doi: 10.1371/journal.pone.0123702

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© 2015 Johnston et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Transformation efficiency of the glnR::kan ^22C cassette into wildtype (wt) recipient. Transformation efficiencies calculated from triplicate repeats. Donor strain, R3154; wt recipient strain, TD198. (B) Transformation efficiency of the codY::trim cassette present in the glnR codY (fecE) mutant into wt and mutant recipient strains. Transformation efficiencies calculated as in panel A. Donor strain, TD196 (i.e. TK108 but rpsL41). Recipient strains, wt, TD198; glnR ^-, TD195; glnR ^- fecE ^-, TD227; fecE ^-, TD230; socY, TD142. (C) Transformation efficiency of the codY::trim cassette from the suppressed codY::trim, socY strain into a wt recipient. Transformation efficiencies calculated as in panel A. Donor strain, TD80; wt recipient as in panel A. (D) Inactivation of fatC and fecE has the same suppressive effect on codY inactivation. Transformation efficiency of the codY::trim cassette present in the TK108 mutant into wt and mutant recipient strains. Transformation efficiencies calculated as in panel A. Donor strain, TD196. Recipient strains, fecE ^-, TD230; fatC ^-, TD141; fecE ^- amiC ^-, TD228; socY, TD142.