Figure 4.

The development of PSC/iPSC expansion substrates: advancing from a complex, chemically undefined, feeder layer-based system to simple, synthetic polymeric substrates with improved efficiency, scalability, and reproducibility

(A) MEF feeder layers support PSC adhesion and self-renewal via their specific secretome contents. (B) ECM-coated substrates composed of an undefined mixture of ECM proteins such as Matrigel™. (C) Surface-tethered functional epitopes derived from ECM components such as E-cadherin and vitronectin (VN)-derived peptides [e.g. heparin-binding peptide, GKKQRFRHRNRKG (Klim et al, 2010)]. (D) Synthetic, polymeric substrates support the attachment and self-renewal of iPSCs via interface-mediated adsorption of essential adhesive ECM components from the culture medium. Examples for such substrates include the following: (1) ultraviolet-/ozone-modified TCPS; (2) poly(methyl vinyl ether-alt-maleic anhydride); (3) poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH). Figure adapted and modified with permission from Celiz et al (2014).