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. 2015 Feb 25;14(3):334–344. doi: 10.1111/acel.12259

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© 2014 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig 3 — Caloric restriction reduced the oxidative stress suffered by SAMP8 astrocytes. (A) SAMP8 astrocytes generated more reactive oxygen species (ROS) than SAMR1 astrocytes in control conditions as measured by increased dichlorofluorescein (DCF) fluorescence units (FU), whereas differences between the two cell types were minor in the presence of hydrogen peroxide. (B) Caloric restriction (CR) reduced the oxygen consumption of the astrocyte cells compared with control conditions (AL). (C) CR barely decreased the excess ROS generated by SAMP8 astrocytes, whereas the decrease it induced in the ROS generated by hydrogen peroxide was only significant in control SAMR1 astrocytes. (D) CR reduced the increased level of carbonylated proteins of SAMP8 astrocytes, as shown in representative oxyblots. Coomassie blue staining was used to normalize protein oxidation in an integrated area including the two main oxidation bands (arrows). Statistics: *P < 0.05 vs. SAMR1; #P < 0.05, and ##P < 0.01, vs. control ad libitum (AL) conditions by Bonferroni's test.