Fig 1.

Experimental design of the pedigree assay. Virgin mothers (shown as initial mother in the figure) were subjected individually to replicative lifespan, and their 5th and last daughters isolated to give rise to individual clones. Virgin mothers from these clones were again subjected to the replicative lifespan procedure, isolating either 5th (from the ‘young’ lineage) or last (from the ‘old’ lineage) daughters. This procedure was repeated six times for four independent WT and four independent Δ8 lines, followed by genome sequencing of the respective colonies following the 2nd, 4th, and 6th cycles (shown by red squares). In total, 48 genomes of individual cell-derived lineages were sequenced and analyzed.