Fig 6.

Follicle-stimulating hormone-induced pro-lipogenic gene expression via Gαi/Ca²⁺-dependent CREB/PPARγ pathway in 3T3-L1 preadipocytes. (A) Total CREB and p-CREB expressions in 3T3-L1 cells treated with FSH (0–100 ng mL⁻¹) for 30 min. (B) PPARγ expression in FSH-induced 3T3-L1 cells at 2–8 days of differentiation with or without pertussis toxin (PTX) (100 nm) treatment. (C) FSHR, CREB and p-CREB expressions in 3T3-L1 cells treated with FSH for 30 min with or without FSHR-specific siRNA treatment. (D) CREB and p-CREB expression in 3T3-L1 cells treated with FSH for 30 min with PTX (100 nm) or verapamil (20 μm). (E) Working model of FSH-induced lipid biosynthesis in adipocytes. Values are mean ± SEM (n = 5 for A–D). *P < 0.05 and **P < 0.01.