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. 2015 Feb 26;14(3):474–482. doi: 10.1111/acel.12329

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© 2015 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig 6 — Western blotting of mTORC1 substrates rpS6 and 4E-BP1 in skeletal muscle, heart, and liver of Snell and Con. In skeletal muscle, rps6 phosphorylation was not different between Snell and Con or between sexes. rpS6 phosphorylation was significantly decreased in heart and liver, which was conserved in both sexes (B, C). There was a slight interaction trend in skeletal muscle (P = 0.098) 4E-BP1 between treatment (Snell) and sex (D). No changes in 4E-BP1 were found in skeletal muscle, heart, or liver between Snell and Con (E, F). n = 5 per sex and n = 10 per group. *P < 0.05 for Snell vs. Con.