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. 2015 Apr 22;10(4):e0123106. doi: 10.1371/journal.pone.0123106

Fig 2. Individual yeast clones identified as potential interacting partners of KIRREL3-ECD and KIRREL3-ICD.

Fig 2

(A) Positive control: yeast mating of [pGBKT7-p53] in AH109 and [pGADT7-T] in Y187. (B) Negative control: yeast mating of [pGBKT7- KIRREL3- ECD] in AH109 and [pGADT7-T] in Y187. (C) Yeast mating of [pGBKT7-KIRREL3-ECD] in AH109 and [pGADT7-MAP1BLC1] in Y187. (D) Yeast mating of [pGBKT7-KIRREL3 ECD] in AH109 and [pGADT7-MYO16] in Y187. (E) Negative control: yeast mating of [pGBKT7- KIRREL3-ICD] in AH109 and [pGADT7-T] in Y187. (F) Yeast mating of [pGBKT7-KIRREL3-ICD] in AH109 and [pGADT7-ATP1B1] in Y187. (G) Yeast mating of [pGBKT7-KIRREL3-ICD] in AH109 and [pGADT7-UFC1] in Y187. (H) Yeast mating of [pGBKT7-KIRREL3-ICD] in AH109 and [pGADT7-SHMT2] in Y187. Yeast mating was performed and cells were grown on—AHLT X-α-gal plates. Only clones with interacting proteins grow on—AHLT X-α-gal media and turn blue.