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. 2015 Apr 22;11(4):e1004851. doi: 10.1371/journal.ppat.1004851

Fig 1. ASD4 is involved in nitrogen assimilation.

Fig 1

(A) Δasd4 mutant strains were grown for 10 days on minimal media (MM) containing 1% (w/v) glucose (GMM) and 10 mM of the indicated sole nitrogen sources. L-isomers were used throughout this study. (B) After 10 days, Δasd4 growth was not further attenuated on GMM containing low concentrations of ammonium as the sole nitrogen. (C) Strains were grown for 10 days on MM containing 10 mM L-glutamine as the sole carbon and nitrogen source. WT = wild type Guy11 isolate, Δasd4 ASD4GFP = Δasd4 complementation strain expressing Asd4 fused to GFP. Bars are added as a visual aid to the plate images in (B) and (C) to demarcate colony size.