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siRNA gradient hydrogel fabrication and characterization. (a) Photograph of the mixing apparatus used to fabricate gradient hydrogels. (b) Flow profiles as programmed into the syringe pumps to create 4 cm siRNA gradient hydrogels. (c) Schematic of the cell-encapsulated siRNA gradient hydrogel construct, containing an siRNA nanoparticle gradient and a uniform cell density. (d) Quantification of siRNA in sequential 0.5 cm segments of siRNA gradient hydrogels.
