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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Circ Cardiovasc Genet. 2015 Jan 22;8(2):284–293. doi: 10.1161/CIRCGENETICS.113.000587

Figure 4.

Figure 4

Effects of Gata6 carboxyl zinc-finger deletion in the developing mouse AV conduction system. In situ hybridization with probes against Gata6-exon4, Tbx3 and Id2 mRNA in frontal sections from Mlc2v-CreGata6f/f mouse embryos are shown. In the absence of Gata6 with a carboxyl zinc-finger, Gata6 expression at E12.5 (A) overlaps with that of Tbx3 (D) and Id2 (G), but the region stained by each of these transcripts is smaller compared to similar anatomic sections from hearts with intact Gata6. A reduced area stained by Gata6 (B), Tbx3 (E) and Id2 (H) persists at E14.5, as well at E16.5 (C, F & I). The white arrows point to regions of the developing CCS that are either marked or devoid of Gata6+ staining. The yellow arrowheads point to regions of positive signal within the developing AV node and AV bundle. The white arrowheads point to regions of positive signal within the developing bundle branches. Scale bar=100 μm. Scale bar in (A) applies to all E12.5 images; scale bar in (B) applies to all E14.5 images; scale bar in (C) applies to all E16.5 images.