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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Circ Cardiovasc Genet. 2015 Jan 22;8(2):284–293. doi: 10.1161/CIRCGENETICS.113.000587

Figure 5.

Figure 5

Gata6 carboxyl zinc-finger deletion reduces HCN4 expression in the AV node. Immunohistochemical staining of frontal sections from mature murine hearts against HCN4 (green) in control hearts (A-E), and Gata6 mutant hearts (F-J), shows HCN4 expression is significantly diminished within the AV node in the absence of Gata6 with a carboxyl zinc-finger (A & F). The dotted yellow line in panel F represents the region of TBX3+ staining in an adjacent section as shown in Supplemental Figure 2. In both the presence and absence of Gata6 with a carboxyl zinc-finger, HCN4-positive cells are expressed in the lower nodal region and portions of the AV bundle (B-D & G-I). The expected lack of HCN4 staining in the bundle branches is seen in the control and mutant hearts (E & J). Blue arrowheads point to the lower nodal region; white arrowheads point to HCN4+ staining in the AV bundle. Scale bar=250 μm and applies to all panels. AVN=atrioventricular node; AVB=atrioventricular bundle; BB=bundle branches; LNR=lower nodal region.