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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Circ Cardiovasc Genet. 2015 Jan 22;8(2):284–293. doi: 10.1161/CIRCGENETICS.113.000587

Figure 6.

Figure 6

Gata6 carboxyl zinc-finger deletion does not affect CX40 expression in the mouse CCS. Immunohistochemical staining of frontal sections from mature murine hearts against connexin40 (CX40, red) in Gata6f/f hearts (A-E) and those without the Gata6 carboxyl zinc-finger (F-J) reveals the expected lack of CX40 staining in the AV node, despite the presence of Gata6 (A & F). In the presence and absence of Gata6 with a carboxyl zinc-finger, the expected expression of CX40 within the AV bundle is observed (B-D & G-I). Deletion of the Gata6 carboxyl zinc-finger does not appear to effect the expression of CX40-positive cells within the bundle branches (E & J). The blue arrowheads point to the lower nodal region; the yellow arrowheads point to regions of CX40+ staining in the bundle branches. Scale bar=250 μm and applies to all panels. AVN=atrioventricular node; AVB=atrioventricular bundle; BB=bundle branches; LNR=lower nodal region.