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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Circ Cardiovasc Genet. 2015 Jan 22;8(2):284–293. doi: 10.1161/CIRCGENETICS.113.000587

Figure 7.

Figure 7

Gata6 carboxyl zinc-finger deletion reduces ID2 expression and cardiomyocyte cell-cycle exit in the developing AV bundle. Immunohistochemical staining in adult murine hearts against ID2 in Gata6f/f hearts (A & C) shows ID2 expression is higher in the proximal CCS compared to the working myocardium. In the absence of Gata6 with a carboxyl zinc-finger (B & D), ID2 expression is reduced in the AV node and AV bundle. Panels E-K show Gata6 with a carboxyl zinc-finger is required for cell-cycle exit of cardiomyocytes in the developing AV bundle. Immunohistochemical staining of E12.5 wild-type hearts (E-G) against PCNA (red) and TBX3 (green), as well as similarly staged Gata6 mutant hearts (H-J). The dashed yellow outline indicates the TBX3-expressing AV bundle. Note there are fewer PCNA/TBX3-positive cells in the Gata6f/f heart compared to the mutant heart. (K) PCNA-positive nuclei in the crest of the septum that overlap with TBX3-expressing cells averaged from five hearts of each genotype. *P<0.01. Scale bar=100 μm. Scale bar in (A) applies to (A-D); scale bar in (E) applies to (E-J). AVN=atrioventricular node; AVB=atrioventricular bundle.