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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Circ Cardiovasc Genet. 2015 Jan 22;8(2):284–293. doi: 10.1161/CIRCGENETICS.113.000587

Figure 8.

Figure 8

GATA6 transactivates Id2 and Ncx1. (A) VISTA comparison of the murine and human Id2 proximal promoter and exon 1, and (B) of the murine and human Ncx1 proximal promoter and exon 1. In panels A and B the x and y axes indicate sequence length (kb) and percent homology (≥ 75%, pink), respectively. GATA6 binding sites conserved in the mouse and human sequence are indicated by asterisks. (C) Schematic of the Id2-LUC reporter containing the 5.2-kb proximal promoter upstream of firefly luciferase (LUC), and (D) the Ncx1-LUC reporter containing the 5.1-kb proximal promoter that is also upstream of firefly luciferase. (E) Activation of the Id2-LUC reporter by GATA6. HL-1 cells were transiently transfected with Id2-LUC and 125–500 ng of expression plasmid encoding wild-type GATA6 or GATA6 lacking exon 4 (Gata6Δexon4). The reporter is activated by wild-type GATA6 but not by GATA6Δexon4. (F). Activation of the Ncx1-LUC reporter by GATA6. HL-1 cells were similarly transiently transfected with Ncx1-LUC and 125–500 ng of expression plasmid encoding wild-type GATA6 or GATA6Δexon4. As with Id2-LUC, the Ncx1-LUC reporter was activated by the expression of wild-type GATA6 but not by GATA6Δexon4. The transient transection studies in panels E and F show the mean change in report activity from at least 3 different experiments at each concentration of expression plasmid.