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In vivo binding of Myc-5 to the E-box at its target gene promoter. (a, b) Schematic depiction of the Myc-5 target gene promoter with MYC binding site (underline) indicated. (c–f) ChIP assay of Myc-5 target genes in the P493.6 (c, d) and K562 (e, f) cell systems. Labeled regions (E-box and exon) of each gene were quantitatively amplified by real-time PCR. Data are representative of three independent experiments. Tet, tetracycline.
