Figure 2.
Expression of GRK2, GRK3, and GRK5 in cardiomyocytes isolated from non-ischemic area 7 days post-MI (A–C). Spearman’s rank correlation identified a significant positive correlation between GRK2 expression level and AdipoR1 phosphorylation 1, 3 and 7 days post-MI (D). N=5/group. **P<0.01 vs. Sham. Intramyocardial injection of siRNA effectively inhibited GRK2 overexpression Post-MI (E) and blocked post-MI AdipoR1 phosphorylation (F). Cardiomyocyte-specific GRK2 deletion blocked AdipoR1 phosphorylation (G). Representative blots from 5 repeated experiments.