Figure 6.
Cardiomyocytes from remote non-infarct regions were isolated 7 days post-MI. Cells were treated with TNFα in the presence or absence of gAPN for 8 hours. gp91phox/iNOS expression (A–D), superoxide production (A/B) and peroxynitrite formation (C/D) were determined. N=5 mice/group. *P<0.05, **P<0.01 vs. TNFα alone, $$P<0.01 vs. sham. E/F: Acute administration of gAPN (intraperitoneal bolus) caused significant GLUT4 membrane translocation (E) and eNOS phosphorylation (F) in control hearts, but these responses were significantly decreased 7 days post-MI. N=6–8 animals/group. *P<0.05, **P<0.01 vs. vehicle; $P<0.05, $$P<0.01 vs. sham with the same treatment.