. 2015 Apr 7;3(4):apps.1400120. doi: 10.3732/apps.1400120

Table 2.

Characteristics of 23 nuclear microsatellite loci developed in this and other studies for Salix humboldtiana.

					RNo03^b		RNo13^b
Locus^a	N	A	A_R	A_e	H_o	H_e^c	H_o	H_e^c
Shum_002	60	4	3.98	2.42	0.517	0.563^ns	0.677	0.598^ns
Shum_006	36	3	2.61	1.73	—	—	—	—
Shum_029	31	2	2.00	1.29	—	—	—	—
Shum_032	60	4^‡	3.85	1.40	0.103	0.267**	0.355	0.301^ns
Shum_033	53	3	3.00	1.92	0.680	0.473^ns	0.786	0.477***
Shum_047	60	7	6.83	2.33	0.414	0.555^ns	0.516	0.571^ns
Shum_049	32	3	2.69	1.21	—	—	—	—
Shum_060	53	6	5.96	2.10	0.542	0.531^ns	0.586	0.505^ns
Shum_061	56	5	5.00	3.09	0.654	0.637^ns	0.667	0.701^ns
Shum_062	31	4	3.42	2.13	—	—	—	—
Shum_064	53	3	2.98	2.00	0.458	0.510^ns	0.517	0.490^ns
Shum_066	60	8	7.73	2.46	0.448	0.488^ns	0.613	0.668^ns
Shum_067	32	4	3.37	1.29	—	—	—	—
Shum_070	59	4	3.76	1.17	0.071	0.070^ns	0.194	0.203*
Shum_071	59	3	2.88	1.44	0.321	0.275^ns	0.226	0.331^ns
Shum_074	59	3	3.00	1.15	0.036	0.035^ns	0.226	0.207^ns
Shum_076	58	5	4.78	1.19	0.071	0.135**	0.200	0.186^ns
Shum_077	52	5	5.00	1.30	0.083	0.081^ns	0.393	0.340^ns
Sa54A	60	7	6.86	2.74	0.414	0.508^ns	0.710	0.721^ns
gSIMCT24	43	7	5.24	1.49	—	—	—	—
ORPM446	22	3	3.00	1.50	—	—	—	—
SB196	29	3	3.00	1.64	—	—	—	—
WPMS18	32	6	5.71	1.73	—	—	—	—

Note: A = number of alleles; A_e = effective number of alleles; A_R = allelic richness standardized by rarefaction; H_e = expected heterozygosity; H_o = observed heterozygosity; N = number of individuals analyzed.

Previously developed loci: Sa54A (King et al., 2010); gSIMCT24 (Stamati et al., 2003); ORPM446 (Tuskan et al., 2004); SB196 (Barker et al., 2003); WPMS18 (Smulders et al., 2001).

Values of H_e and H_o are not shown for loci evaluated with a panel of individuals that were not part of populations RNo03 or RNo13.

Significant deviations from Hardy–Weinberg equilibrium: *P < 0.05; **P < 0.01; ***P < 0.001; ns = not significant.

^‡

Locus showing null allele at one of the analyzed populations.