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. Author manuscript; available in PMC: 2016 Apr 28.
Published in final edited form as: FEBS Lett. 2015 Mar 18;589(10):1143–1149. doi: 10.1016/j.febslet.2015.03.010

Figure 2. Expression of miR-184 in the retina of OIR mice and regulation of Wnt signaling by miR-184.

Figure 2

C57BL/6J mice were under room air (normoxia control) or in the oxygen chamber from P7 to P12 (OIR model). Expression levels of miR-184 in the retinas at P16 were evaluated by qRT-PCR (A). MiR-184 mimic and its control (25 nM each) or miR-184 inhibitor and its control (50 nM each plus 5 nM miR-184 mimic) were separately transfected into rMC1-TOP cells stably expressing firefly luciferase under the control of the Wnt/β-catenin system. The cells were treated with W3A-conditioned medium (10%) at 32 hr post-transfection and Wnt signaling activity assay (TOP flash assay) was conducted at 48 hr post-transfection (B).