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. Author manuscript; available in PMC: 2016 Apr 20.
Published in final edited form as: Dev Cell. 2015 Apr 9;33(2):125–135. doi: 10.1016/j.devcel.2015.02.021

Figure 1. Transient Sox2+ stem cells contribute to all epithelial cell lineages during molar development.

Figure 1

(A) Timing of tamoxifen induction and sample harvest in Sox2-CreER;R26R mice. (B-D) LacZ expression assayed by X-gal staining (blue) in sagittal sections of the lower first molar 48 hr (B) and 1 week (C,D) after tamoxifen induction. Broken line in (B) indicates basement membrane. Boxed area in (C) is shown magnified in (D). X-gal staining is detectable in dental epithelial cells, but not in the dental mesenchyme. Note that all lacZ+ epithelial cell types in the enamel organ (D), including AM, OEE, SR, and SI, are derived from Sox2+ cells. (E-J) Immunofluorescence of Sox2 (green) in sagittal sections of the lower first molar and incisor at E16.5 (E-G) and PN0.5 (H-J). Boxed areas in (E) and (H) are shown magnified in (F/G) and (I/J), respectively. Broken lines indicate cervical loop areas. Note the absence of Sox2 expression (white arrow, I) in mouse molars at PN0.5. AM: Ameloblast; DE: Dental epithelium; DM: Dental mesenchyme; DP: Dental papilla; IN: Incisor; M1: Lower first molar; M2: Lower second molar; OEE: Outer enamel epithelium; SI: Stratum intermedium; SR: Stellate reticulum. Scale bars (B, D, F, G, I, J): 50μm. Scale bars (C, E, H): 200μm. See also Figure S1.