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. Author manuscript; available in PMC: 2016 Apr 20.
Published in final edited form as: Dev Cell. 2015 Apr 9;33(2):125–135. doi: 10.1016/j.devcel.2015.02.021

Figure 2. Loss of Smad4 in the dental epithelium prolongs the maintenance of the cervical loop and molar crown development.

Figure 2

(A) Timing of doxycycline induction and sample harvest in control and KRT14-rtTA;tetO-Cre;Smad4fl/fl (K;T;S) mice. (B-E) Macroscopic views (B), quantitation of the crown length (C) and H&E staining (D, E) of PN21.5 control and KRT14-rtTA;tetO-Cre;Smad4fl/fl lower first molars. Arrows indicate crown length. N=3. *, p < 0.05. Error bars indicate standard deviation (SD). (F-Q) Time-course analysis of H&E staining (F, G, J, K, N, O) and Keratin 14 (K14; green) immunofluorescence (H, I, L, M, P, Q) of control and KRT14-rtTA;tetO-Cre;Smad4fl/fl lower first molars at PN7.5 (F-I), PN12.5 (J-M), and PN21.5 (N-Q). Arrows indicate the HERS in control lower first molars. Broken lines indicate the cervical loop in KRT14-rtTA;tetO-Cre;Smad4fl/fl lower first molars. M1: Lower first molar; M2: Lower second molar; R: Root. Scale bars (D, E): 500μm. Scale bars (F-Q): 50μm.